关于细胞身份验证启事

发表日期:2012/11/2 20:59:00    浏览数:2463

自2009年7月1日起,美国癌症研究协会的杂志对投稿文章所使用的细胞提出了身份验证的要求。要求在文章的方法与材料部分,详细介绍所使用细胞情况,包括: 1.     From where and when the cells were obtained

2.     Whether the cell lines have been tested and authenticated

3.     The method by which the cells were tested

4.     How and when the cells were last tested

If cells were obtained directly from a cell bank that performs cell line characterizations and passaged in the user!@$%s laboratory for fewer than 6 months after receipt or resuscitation, re-authorization is not required. In these cases, please include the method of characterization used by the cell bank.

AACR Journals: •         Cancer Research

•         Clinical Cancer Research

•         Cancer Epidemiology, Biomarkers & Prevention, CEBP

•         Cancer Prevention Research

•         Molecular Cancer Research

•         Molecular Cancer Therapeutics

•         Cancer Reviews Online

•         Prevention Portal

1、使用细胞:请参照 UKCCCR Guidelines for the Use of Cell Lines in Cancer Research, British J of Cancer (2000) 82(9); 1495–1509.

2、 细胞身份验证:请参照   Cell Line Verification Test Recommendations, ATCC Technical Bulletin No. 8 (2008). 主要是细胞生长情况、支原体检测、种属鉴定、STR检测。

3、 细胞身份验证方法:请参照

    Cabrera CM et al., Identity Test: Determination of Cell Line Cross-Contamination, Cytotechnology (2006) 51:45–50.

    Masters JR et al., Short Tandem Repeat Profiling Provides an International Reference Standard for Cell Lines, PNAS (2001) 98(14):8012–17.


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